Engineering polyelectrolyte multilayer coatings as a strategy to optimize enzyme immobilization on a membrane support

This study presents a systematic design of biocatalytic membrane reactor, where we combined physical adsorption and chemical conjugation Alcohol Dehydrogenase (ADH) in novel type polyelectrolyte (PE) layer-by-layer (LbL) assembly system. The hybrid LbL structure is proposed as strategy to simultaneously advance activity operational stability enzymes immobilized on surface. Using poly(allylamine hydrochloride) (PAH) poly(methacrylic acid) sodium salt (PMAA) allowed functionalization polysulfone (Psf) for subsequent ADH immobilization with no resistance substrate mass transfer decrease water permeability (WP) (56.5 ± 8.8 Lm−2h −1bar−1) compared the pristine (50.8 1.7 Lm−2h−1bar−1). Tailoring surface chemistry, enzyme concentration, time, pH during step increase specific from 1.13 0.18 mU/cm2 4.09 0.53 PAH-modified coating. Conjugation adsorbed PMAA layer improved reusability non-conjugated (by retaining 73.4 3.2% starting third conversion run against 58.7 2.4%) shifted optimum by 1 unit free ADH. presented approach synthesis provides potential foundation engineering nanoscale reactors.